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Huis> Blog> What is Real-Time PCR Instrument?

What is Real-Time PCR Instrument?

September 22, 2023

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A Real-Time PCR Instrument, also known as a quantitative PCR (qPCR) instrument, is a laboratory device used to perform the polymerase chain reaction (PCR) technique in real time. PCR is a widely used molecular biology technique that amplifies a specific region of DNA or RNA to generate multiple copies for further analysis.

Real-time PCR instruments combine thermal cycling and fluorescence detection capabilities to monitor the amplification of the target nucleic acid during each PCR cycle. Unlike traditional PCR, where the amplification is examined at the end of the reaction, real-time PCR allows for continuous monitoring of the amplification in real time.

Real-time PCR instruments rely on the use of fluorescent dyes or specialized probes that emit fluorescence when they bind to the target DNA or RNA. As the amplification progresses, the instrument measures the increasing fluorescence signal, which is proportional to the amount of target nucleic acid present in the reaction. This enables the precise quantification and analysis of the target molecule.

Real-time PCR instruments offer several advantages over traditional PCR, including:

1. Quantitative Analysis: Real-time PCR allows for accurate, quantitative measurement of the initial amount of target nucleic acid present in the sample.

2. Increased Sensitivity: The continuous monitoring of amplification enables the detection of low-abundance targets with higher sensitivity.

3. Faster Results: Real-time PCR reduces the time required for PCR analysis by eliminating the need for gel electrophoresis and post-PCR analysis.

4. Wide Range of Applications: Real-time PCR instruments are used in various applications, including gene expression analysis, pathogen detection, quantitative nucleic acid analysis, genetic variation analysis, and more.

Real-time PCR System instruments are available in different formats and capacities, ranging from small desktop instruments for low-throughput applications to high-throughput systems capable of processing hundreds of samples simultaneously. They are essential tools in molecular biology research, diagnostics, and other scientific applications involving nucleic acid analysis.

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