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Huis> Blog> what is 4-channel Fluorescence quantitative Real time PCR?

what is 4-channel Fluorescence quantitative Real time PCR?

February 27, 2024

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A 4-channel fluorescence quantitative real-time PCR (polymerase chain reaction) system is a molecular biology technique used to amplify and quantify targeted DNA sequences in a sample. This technology allows for the simultaneous detection of four different fluorophores during the amplification process. Each channel corresponds to a specific fluorescent dye that can be used to detect the presence and accumulation of specific DNA sequences or genetic markers.

In traditional PCR System, a single dye, such as SYBR Green, or a specific labeled probe, is used to monitor the accumulation of amplified DNA during each cycle of the PCR reaction. However, with a 4-channel system, researchers have the capacity to monitor multiple targets within the same reaction. This is particularly useful for multiplex assays, where several different DNA targets can be amplified and detected in a single reaction, providing a more efficient and cost-effective method for analyzing multiple genetic targets at once.

The ability to run multiplex assays in a 4-channel fluorescence quantitative real-time PCR system offers several advantages, including increased throughput, reduced sample consumption, and the ability to analyze complex genetic profiles in a single experiment. This technology is widely used in various fields, including gene expression analysis, genotyping, pathogen detection, and other molecular biology applications where the simultaneous detection of multiple genetic targets is essential.

These systems are equipped with sophisticated optics, detectors, and software to capture and analyze the fluorescent signals emitted during the PCR process. This allows researchers to monitor the amplification of multiple targets in real time, providing accurate quantification and analysis of the genetic material present in the sample.

Overall, 4-channel fluorescence quantitative RT-qPCR Instrument plays a critical role in advancing research and diagnostics by providing a powerful platform for high-throughput, multiplexed DNA analysis, thus enabling comprehensive genetic profiling and precise quantification of different DNA targets within a single experiment.

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