The Specification of Sanger sequencer
November 10, 2023
The specifications of a Sanger sequencer can vary depending on the specific model and manufacturer. However, here are some common specifications typically associated with a Sanger sequencer:
1. Throughput: The throughput of a Sanger sequencer refers to the number of sequencing reactions it can process simultaneously. This can range from a few samples to hundreds of samples, depending on the specific model.
2. Read length: The read length of a Sanger sequencer refers to the maximum length of DNA sequence that can be reliably determined. The typical read lengths range from a few hundred bases to over 1,000 bases, depending on the specific model and chemistry used.
Genetic Analyzer Display
3. Accuracy: Sanger sequencing is known for its high accuracy, with error rates typically below 1 in 1,000 nucleotides. The specific accuracy may vary slightly depending on the chemistry and protocols used.
4. Instrument footprint: The physical size of a Sanger sequencer can vary, but most benchtop models have a compact footprint that fits on a laboratory bench or in a hood.
5. Instrument weight: The weight of a DNA Sequencer can vary depending on the specific model. Benchtop models are typically lightweight and portable.
6. Sample compatibility: Sanger sequencers can handle a variety of DNA sample types, including genomic DNA, cDNA, PCR products, and plasmids. The specific compatibility may depend on the specific protocols and kits used.
7. Electrophoresis: Sanger sequencers utilize capillary electrophoresis for separating DNA fragments based on size. The capillary electrophoresis system may vary in terms of capillary length, number of capillaries, and detection technology (e.g., fluorescence).
8. Software and data analysis: Sanger sequencer systems typically come with software for instrument control and data analysis. The software allows for base calling, sequence alignment, and quality assessment of the sequencing data.
9. User interface: The user interface of a Sanger Sequecing Instrument can vary but typically includes a touchscreen or computer interface for easy instrument control and data analysis.
It is important to note that these specifications can vary between different models and manufacturers, and advancements in technology may lead to improvements and new features in Sanger sequencers.
