The Specification of Real Time Quantitative PCR System
October 18, 2023
PCR System Display
The specifications of a real-time Quantitative PCR System can vary depending on the specific instrument and manufacturer. However, here are some common specifications that you may find in a typical qPCR system:
1. Temperature range: The qPCR system should be able to control temperature accurately within a specified range, typically ranging from around 4°C to 99°C. This range allows for accurate denaturation, annealing, and extension of the DNA during the PCR process.
2. Sample capacity: The system should have a capacity to hold a certain number of samples. This can vary from a few samples to 96 or 384-well plate formats, allowing for high-throughput analysis.
3. Optical detection: The qPCR system should have the ability to detect fluorescence signals emitted from fluorophore-labeled probes or DNA-binding dyes during the amplification process. It typically includes multiple channels or filters for detecting different fluorophores.
4. Excitation sources: The system may use different excitation sources, such as LED (Light-Emitting Diode) or laser, to excite the fluorophores. LED sources are commonly used due to their cost-effectiveness and durability.
5. Detection sensitivity: The sensitivity of the qPCR system refers to its ability to detect and quantify low amounts of nucleic acids accurately. This is typically expressed as the minimum number of copies of the target nucleic acid that can be reliably detected.
6. Dynamic range: The dynamic range of the qPCR system specifies the linear range over which the system can accurately quantify the template concentration. It is typically expressed as a range of template copy numbers, such as from 10 to 10^9 copies.
7. Software and data analysis: The Quantitative PCR Machine should come with dedicated software that allows for easy setup and control of the qPCR process, as well as automated data analysis and result visualization. This software may include features such as threshold cycle (Ct) determination, melting curve analysis, and data export options.
8. Connectivity and compatibility: The system may have options for connectivity and compatibility with other instruments, such as robotic systems, liquid handling platforms, or data management systems, to facilitate automation and integration into larger workflows.
It is important to note that the specifications can vary between different models and manufacturers. Checking the specific specifications of the instrument from the manufacturer's documentation or website is essential when considering a qPCR system for a particular application.
